Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

180
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
180
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Integrins01:10

Integrins

4.2K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
4.2K
LTR Retrotransposons03:08

LTR Retrotransposons

18.0K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.0K
Retroviruses02:33

Retroviruses

12.7K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.7K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cryoelectron tomography of HIV-1 cell-cell transmission conjugates reveals a secluded environment for viral assembly and transfer.

bioRxiv : the preprint server for biology·2026
Same author

A long-chain heparan sulfate capture mechanism directs paracrine GDNF-GFRα1 signalling through RET.

bioRxiv : the preprint server for biology·2026
Same author

Temporal Evolution of Drug Resistance to HIV Integrase Inhibitors.

Viruses·2026
Same author

In situ cryo-ET defines the ultrastructure of ER exit sites in human cells.

Nature cell biology·2026
Same author

intmap: fast and flexible mapping of mobile DNA integration for basic and translational research.

Bioinformatics (Oxford, England)·2026
Same author

Relationship between the distribution of LEDGF along genes and positions of HIV-1 DNA integration.

mBio·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Sep 24, 2025

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

17.8K

Multivalent interactions essential for lentiviral integrase function.

Allison Ballandras-Colas1,2, Vidya Chivukula1,3, Dominika T Gruszka4,5

  • 1Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.

Nature Communications
|May 3, 2022
PubMed
Summary
This summary is machine-generated.

Maedi-visna virus (MVV) integrase (IN) forms a large intasome complex critical for viral DNA integration. This structure facilitates interactions with host factor LEDGF/p75, guiding integration into active genes.

More Related Videos

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Published on: April 9, 2014

18.0K
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

379

Related Experiment Videos

Last Updated: Sep 24, 2025

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

17.8K
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Published on: April 9, 2014

18.0K
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

379

Area of Science:

  • Virology
  • Structural Biology
  • Molecular Biology

Background:

  • Retroviral integrase (IN) forms intasome complexes to integrate viral DNA into host genomes.
  • Maedi-visna virus (MVV), an ovine lentivirus, utilizes a large intasome assembly with sixteen IN subunits.

Purpose of the Study:

  • To determine the cryo-electron microscopy (cryo-EM) structures of the lentiviral intasome before and after target DNA engagement.
  • To elucidate the protein-protein and protein-DNA interfaces essential for intasome formation and function.
  • To investigate the role of the intasome's architecture and host factor LEDGF/p75 in viral integration.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) to resolve intasome structures at 3.4 and 3.5 Å resolution.
  • In vitro biochemical assays to assess integrase strand transfer activity.
  • Single-molecule microscopy with photobleaching to study LEDGF/p75 binding.
  • Cellular assays to observe MVV integration site distribution upon LEDGF/p75 ablation.

Main Results:

  • Cryo-EM structures revealed detailed interfaces of the lentiviral intasome.
  • Specific IN homomeric interfaces and linker domain configuration are crucial for strand transfer and infectivity.
  • The MVV intasome binds variable numbers of LEDGF/p75 (up to sixteen molecules).
  • Ablation of LEDGF/p75 significantly alters MVV integration site distribution in host cells.

Conclusions:

  • The expanded architecture of lentiviral intasomes is vital for their function.
  • Multivalent interactions with chromatin, mediated by LEDGF/p75, likely direct integration into active genes.
  • Understanding these interactions provides insights into lentiviral replication and potential therapeutic targets.