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Updated: Sep 24, 2025

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
Published on: May 5, 2023
Reprogramming Mouse Oviduct Epithelial Cells Using In Vivo Electroporation and CRISPR/Cas9-Mediated Genetic
Matthew J Ford1, Yojiro Yamanaka2
1Department of Human Genetics, Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, Quebec, Canada.
Abstract:
Advances in gene editing tools such as CRISPR/Cas9 have made precise in vivo gene editing possible, opening up avenues of research into somatic cell reprograming to study adult stem cells, homeostasis, and malignant transformation. Here we describe a method for CRISPR/Cas9 mediated in vivo gene editing, in combination with Cre-based lineage tracing via electroporation in the mouse oviduct. This method facilitates the delivery of multiple plasmids into oviduct epithelial cells, sufficient for studying homeostasis and generation of high-grade serous ovarian cancer (HGSOC) models.

