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Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
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In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay
Nick A Kuburich1, Petra den Hollander1, Abhijeet P Deshmukh1
1Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2022
Abstract:
Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. In this chapter, we provide a detailed method for the quantification of CSCs in vitro through mammosphere formation.

