Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Ultrasound II: Endoscopic Ultrasound and FibroScan

Ultrasound II: Endoscopic Ultrasound and FibroScan

Endoscopic Ultrasound (EUS) and FibroScan are valuable diagnostic tools in gastroenterology and hepatology, each with specific applications and techniques.
Endoscopic Ultrasound (EUS):

Fibronectins Connect Cells with ECM

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Non-Coding RNAs in Neurological and Neuropsychiatric Disorders: Unraveling the Hidden Players in Disease Pathogenesis.

Cells·2024

Same author

Physiological Reports celebrates its first 10 years.

Physiological reports·2023

Same author

Benchmarking RNA Editing Detection Tools.

Biotech (Basel (Switzerland))·2023

Same author

Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages.

Non-coding RNA·2023

Same author

Methods and Tools in RNA Biology.

Non-coding RNA·2023

Same author

DoxoDB: A Database for the Expression Analysis of Doxorubicin-Induced lncRNA Genes.

Non-coding RNA·2023

Same journal

A non-hydrolyzable candesartan cilexetil analog reveals synergistic activation as a tractable mechanism for TMEM175 modulation.

American journal of physiology. Cell physiology·2026

Same journal

Mitochondrial Calcium Transport in Amino Acid Metabolism: From nutritional responses to metabolic regulation.

American journal of physiology. Cell physiology·2026

Same journal

N-linked glycosylation regulates SNAT2 trafficking and stability in pancreatic ductal adenocarcinoma cells.

American journal of physiology. Cell physiology·2026

Same journal

Oxaloacetate Inhibition of Succinate Dehydrogenase: Mechanism and Physiological Implications.

American journal of physiology. Cell physiology·2026

Same journal

miR-339-5p impairs myogenic proliferation and differentiation by repressing contractile gene programs in skeletal muscle.

American journal of physiology. Cell physiology·2026

Same journal

Impact of ΔF508 CFTR Mutation on Diaphragm Function During Acute Inflammation.

American journal of physiology. Cell physiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 24, 2025

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Published on: July 3, 2025

Epitranscriptomics in fibroblasts and fibrosis.

Mirolyuba Ilieva¹, Shizuka Uchida¹

¹Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark.

American Journal of Physiology. Cell Physiology

|May 4, 2022

Summary

This summary is machine-generated.

Fibroblast-associated fibrosis is modulated by epitranscriptomic marks like RNA methylation and editing. Understanding these RNA modifications offers new therapeutic targets for fibrotic diseases.

Keywords:

RNA editing RNA methylation epitranscriptomics fibroblast fibrosis

More Related Videos

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

Related Experiment Videos

Last Updated: Sep 24, 2025

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

Published on: July 3, 2025

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

Area of Science:

Molecular Biology
Epigenetics
Pathology

Background:

Fibroblasts are key players in fibrotic diseases affecting organs like lungs, heart, liver, skin, and kidneys.
The epitranscriptome, encompassing over 170 RNA modifications, is increasingly recognized for its role in modulating fibrosis.
Recent advancements in high-throughput technologies enable detailed investigation of RNA modification sites and their mechanisms in fibrosis.

Purpose of the Study:

To review the latest research on epitranscriptomic modifications in fibroblast biology and fibrosis.
To highlight the involvement of specific RNA modifications, including N6-adenosine methylation, A-to-I RNA editing, and 5-methylcytosine, in fibrotic processes.
To elucidate future research directions for targeting epitranscriptomic alterations in fibrotic diseases.

Main Methods:

Review of current literature on epitranscriptomics and fibrosis.
Focus on high-throughput methods for identifying RNA modification sites and their functions.
Analysis of mechanisms involving key epitranscriptomic regulators such as METTL3/14/16, FTO, ALKBH5, and ADAR proteins.

Main Results:

N6-adenosine methylation, A-to-I RNA editing, and 5-methylcytosine are prevalent RNA modifications found in mRNA, pre-mRNA, and ncRNAs.
These modifications have been observed to influence fibroblast behavior and contribute to fibrosis in various experimental models.
Specific enzymes and proteins involved in RNA modification (e.g., METTL3, FTO, ADAR) play critical roles in the pathogenesis of fibrosis.

Conclusions:

Epitranscriptomic modifications are significant modulators of fibroblast function and fibrotic disease progression.
Targeting RNA modifications presents a promising avenue for developing novel therapeutic strategies against fibrosis.
Further research is warranted to fully understand the intricate roles of epitranscriptomic marks in fibrosis and to translate these findings into clinical applications.