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Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses
Fernanda Salvato1, Raquel Figueiredo2,3, Paulo Mazzafera4,5
1Plant and Microbial Biology Department, North Carolina State University, Raleigh, NC, USA.
Abstract:
Nuclei enrichment procedures enable a large variety of investigations. These studies include structural characterization of nuclear proteins, identification of posttranslational modifications, and regulation of stress or development-related gene expression. Successful enrichment of nuclei samples from plant tissues is crucial for a comprehensive analysis of the plant nuclear proteome. Here, we describe a method for nuclei enrichment from sugarcane stems and its assessment by western blot.
