Related Experiment Video

Updated: Sep 24, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

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CDK5RAP3, an essential regulator of checkpoint, interacts with RPL26 and maintains the stability of cell growth

Hongchen Yan1, Jun-Jie Xu1, Ilyas Ali1

  • 1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Cell Proliferation
|May 5, 2022
PubMed
Abstract

Insights

CDK5RAP3 protein is crucial for cell growth stability. It interacts with RPL26 to regulate the mTOR pathway, impacting cell cycle progression and apoptosis, suggesting its potential as a gene therapy target.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • CDK5RAP3 (CDK5 regulatory subunit associated protein 3) is a key gene regulating cell proliferation, apoptosis, invasion, and metastasis.
  • The precise mechanisms underlying CDK5RAP3's functions in cellular signaling pathways remain largely undefined.

Purpose of the Study:

  • To investigate the role of CDK5RAP3 in cell growth, mitosis, and cell death.
  • To elucidate the molecular mechanisms by which CDK5RAP3 influences these cellular processes.

Main Methods:

  • Utilized conditional knockout mice (MEFs) to inhibit CDK5RAP3.
  • Employed knockdown techniques in MCF7 cells to study CDK5RAP3 function.
  • Investigated protein-protein interactions and pathway regulation.

Main Results:

  • CDK5RAP3 is localized in the centrosome, spindle, and endoplasmic reticulum, suggesting broad cellular regulatory roles.
  • CDK5RAP3 deficiency leads to cell growth instability, G2/M cell cycle arrest, and decreased proliferation by downregulating CDK1 and CCNB1.
  • CDK5RAP3 interacts with RPL26, inhibiting the mTOR pathway, inducing autophagy, and increasing apoptosis, which collectively slows cell growth.

Conclusions:

  • CDK5RAP3 interacts with RPL26 to maintain cell growth stability.
  • CDK5RAP3 plays a significant role in regulating cell growth and presents potential as a target for gene medicine.

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