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Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells
Miganoosh Simonian1, Mozhan Haji Ghaffari1, Ali Salimi2
1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Background:
Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.
Methods:
In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines.
Results:
Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.
Conclusion:
Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.
Insights
This study shows that sortilin is present on breast cancer cells and can be targeted by an antibody to induce cancer cell death. This suggests sortilin as a potential therapeutic target for breast cancer.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Sortilin plays a significant role in various malignancies.
- Sortilin presents a promising therapeutic target for cancer eradication.
Purpose of the Study:
- To evaluate sortilin expression in breast cancer cell lines (4T1 and MDA-MB231).
- To assess the potential of anti-sortilin monoclonal antibody (mAb) in inducing apoptosis in these cell lines.
Main Methods:
- Cell surface expression of sortilin was analyzed using flow cytometry.
- Immunocytochemistry was employed to confirm sortilin localization.
- Apoptosis assays were conducted to measure cell death induction.
Main Results:
- Anti-sortilin (2D8-E3) mAb recognized sortilin on 79.2% of 4T1 cells and 90.3% of MDA-MB231 cells.
- Immunocytochemistry confirmed sortilin's surface expression.
- The anti-sortilin mAb successfully induced apoptosis in both cell lines.
Conclusions:
- Surface sortilin is crucial for breast carcinoma cell survival.
- Anti-sortilin mAb demonstrates potential as a therapeutic agent in targeted cancer therapies.
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