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Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
Josie Chrenek1, Rebecca Kirsch2, Kali Scheck2
1Department of Biomedical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada.
STAR Protocols
|May 5, 2022
Summary
This study details a 3D bioprinting protocol for creating neural tissue models. The method uses fibrin bioink and a specific bioprinter, enabling versatile 3D tissue construction for research applications.
Area of Science:
- Biotechnology
- Tissue Engineering
- Additive Manufacturing
Background:
- 3D bioprinting offers a promising approach for fabricating complex tissue constructs that mimic native biological environments.
- Current methods face challenges in reproducibility and scalability for creating functional tissue models.
Purpose of the Study:
- To present a standardized protocol for 3D bioprinting neural tissues.
- To demonstrate the versatility of the protocol for various cell types and bioinks.
- To facilitate the development of advanced 3D tissue models for research.
Main Methods:
- Utilized a CELLINK BIO X bioprinter with a pneumatic printhead.
- Employed Axolotl Biosciences' fibrin-based bioink for neural tissue fabrication.
- Developed a workflow applicable to chemically crosslinked bioinks and diverse cell lines.
Main Results:
- Successfully 3D bioprinted neural tissue constructs.
- Demonstrated the protocol's adaptability for different cell lines and bioinks.
- Established a reproducible method for creating 3D tissue models.
Conclusions:
- The presented 3D bioprinting protocol enables the creation of functional neural tissue models.
- This workflow supports applications in drug screening and disease modeling.
- The method provides a foundation for advancing tissue engineering and regenerative medicine.
