Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

186
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
186
Proteomics01:33

Proteomics

8.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.0K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cleaved SREBP-2 C-terminal fragment noncanonically drives interleukin-1 receptor-associated kinase 1-dependent apoptosis.

Signal transduction and targeted therapy·2026
Same author

Endotrophin- and CD44-Mediated Heterotypic Signaling Mediates Tumor-Stroma Cross-talk and Facilitates Malignant Progression in Hepatocellular Carcinoma.

Cancer research·2026
Same author

Let There Be Light for Photoproximity Labeling.

ACS central science·2025
Same author

Intracristal space proteome mapping using super-resolution proximity labeling with isotope-coded probes.

Nature communications·2025
Same author

Key challenges and recommendations for defining organelle membrane contact sites.

Nature reviews. Molecular cell biology·2025
Same author

Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering.

Nature communications·2025

Related Experiment Video

Updated: Sep 24, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K

Molecular Spatiomics by Proximity Labeling.

Myeong-Gyun Kang1, Hyun-Woo Rhee1

  • 1Department of Chemistry, Seoul National University, Seoul 08826, Korea.

Accounts of Chemical Research
|May 5, 2022
PubMed
Summary
This summary is machine-generated.

Proximity labeling is a powerful chemical technique for mapping biomolecules within live cells and animal models. This method enables the study of local proteomes, RNA, and DNA, advancing the field of spatiomics.

More Related Videos

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

34.9K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

552

Related Experiment Videos

Last Updated: Sep 24, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K
Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

34.9K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

552

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Proximity labeling is an enzymatic reaction that modifies biomolecules within live cells.
  • It allows for the characterization of local proteomes and spatiotemporal interactomes.
  • Conventional methods are limited in accessing such spatially resolved biological information.

Purpose of the Study:

  • To introduce the fundamental concept of proximity labeling.
  • To highlight multidirectional advances in proximity labeling techniques.
  • To encourage further utilization and modification of proximity labeling in research.

Main Methods:

  • Enzymatic "in-cell" chemical reactions for proximity-dependent biomolecule modification.
  • Mass spectrometry for isolation and identification of modified proteins.
  • Development of chemical photocatalysts as artificial enzymes for proximity labeling.
  • Enrichment and sequencing of modified biomolecules to detect labeling events.

Main Results:

  • Successful characterization of local proteomes (e.g., sub-mitochondrial, membrane contact sites).
  • Profiling of local RNA and DNA is achievable with proximity labeling.
  • Elucidation of spatial cell-cell interaction networks in live animal models.
  • In vivo proximity labeling in mouse models yielded liver-specific secretome and muscle-specific mitochondrial matrix proteome.

Conclusions:

  • Proximity labeling is an essential tool for "spatiomics," extracting spatially distributed biological information.
  • The technique has broad applications in biology and chemistry, including in vivo studies.
  • Continued development promises further revelations in tissue-specific molecular localization and cellular interactions.