Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Regulation01:08

Allosteric Regulation

59.4K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
59.4K
Regulation of Metabolism01:19

Regulation of Metabolism

10.0K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
10.0K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.9K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.9K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.4K
2.4K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

5.0K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.0K
Regulated Protein Degradation02:58

Regulated Protein Degradation

7.7K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Local optogenetic control of genome editing and tumorigenesis <i>in vivo</i> using wireless implantable optoelectronics.

bioRxiv : the preprint server for biology·2025
Same author

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation.

Journal of visualized experiments : JoVE·2024
Same author

Development and Application of Rapamycin-regulated Tyrosine Phosphatases.

Journal of visualized experiments : JoVE·2024
Same author

Enhancing ferryl accumulation in H<sub>2</sub>O<sub>2</sub>-dependent cytochrome P450s.

Journal of inorganic biochemistry·2023
Same author

Dissecting protein tyrosine phosphatase signaling by engineered chemogenetic control of its activity.

The Journal of cell biology·2022
Same author

Conformational Dynamics Allows Sampling of an "Active-like" State by Oncogenic K-Ras-GDP.

Journal of molecular biology·2022

Related Experiment Video

Updated: Sep 24, 2025

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

716

Engineered Allosteric Regulation of Protein Function.

Jordan Fauser1, Nicholas Leschinsky1, Barbara N Szynal1

  • 1University of Illinois-Chicago, Department of Cellular and Molecular Pharmacology and Regenerative Medicine, College of Medicine, 835 S Wolcott Ave E403 (MC868), Chicago, IL 60612, United States.

Journal of Molecular Biology
|May 5, 2022
PubMed
Summary
This summary is machine-generated.

Engineered allosteric regulation offers precise control over protein function for studying cell signaling. These advanced bioengineering techniques overcome limitations of traditional methods, enabling new insights into biological events and potential precision medicine applications.

Keywords:
allosterychemogeneticsoptogeneticsprotein engineering

More Related Videos

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

481
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

29.6K

Related Experiment Videos

Last Updated: Sep 24, 2025

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

716
Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

481
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

29.6K

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Protein Engineering

Background:

  • Allosteric regulation is crucial for understanding cell signaling but traditional methods lack spatiotemporal control.
  • Constitutive mutants and inhibitors often cause unintended signaling consequences, complicating data interpretation.

Purpose of the Study:

  • To review recent advancements in engineered allosteric regulation for precise control of protein function.
  • To discuss various bioengineered allosteric techniques, including chimeric GPCRs, chemogenetic, and optogenetic switches.
  • To highlight the benefits, drawbacks, and future directions of these engineered systems.

Main Methods:

  • Protein engineering approaches for controlled allosteric mechanisms.
  • Development of bioengineered allosteric techniques for in vitro and in vivo applications.
  • Review of chimeric GPCRs, chemogenetic switches, and optogenetic switches.

Main Results:

  • Engineered allosteric methods provide high specificity and precise spatial and temporal regulation of protein activity.
  • These techniques offer significant advantages over traditional methods for studying cell signaling.
  • The review details the benefits and limitations of current engineered allosteric approaches.

Conclusions:

  • Engineered allosteric regulation provides powerful tools for dissecting complex biological events.
  • These advanced techniques have the potential to significantly impact the field of precision medicine.
  • Further improvements in engineered allosteric systems will enhance their utility in biological research and therapeutic applications.