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Related Experiment Video

Updated: Sep 24, 2025

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

Microphysiological stem cell models of the human heart.

Ulgu Arslan¹, Alessia Moruzzi^2,3, Joanna Nowacka⁴

¹Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, the Netherlands.

Materials Today. Bio

|May 6, 2022

Summary

Human pluripotent stem cells (hPSCs) offer superior human heart models over animal studies. Advanced 3D models using hPSC-derived cells overcome limitations of simple cultures for studying heart disease and drug responses.

Keywords:

Cardiac microtissue Cardiomyocyte maturation Engineered heart tissue Functional readout Heart-on-chip Human induced pluripotent stem cells Multicellular cell diseases and drug efficacy platform Structural readout

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

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Last Updated: Sep 24, 2025

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Published on: June 16, 2020

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

Area of Science:

Cardiovascular Research
Stem Cell Biology
Pharmacology

Background:

Human pluripotent stem cells (hPSCs) are increasingly utilized for modeling human heart diseases and drug responses due to their superior ability to recapitulate human cardiac function compared to animal models.
Traditional monolayer cultures of hPSC-derived cardiomyocytes exhibit limitations in fully capturing complex cardiac physiology and disease states.

Purpose of the Study:

To review advanced 3D multi-cell-type models that overcome the shortcomings of simple hPSC-derived cardiomyocyte cultures.
To discuss tailored readouts and sensors for monitoring tissue and cellular physiology in these complex models.
To explore the perspectives for implementing these advanced models in both academic research and industrial drug development.

Main Methods:

Review of existing literature on 3D multi-cell-type models derived from human pluripotent stem cells.
Analysis of various ex vivo and in situ monitoring techniques and sensor technologies for physiological measurements.
Discussion of implementation strategies and future directions for advanced cardiac modeling.

Main Results:

3D multi-cell-type models offer enhanced recapitulation of human cardiac (dys-)function compared to 2D cultures.
Development of sophisticated monitoring tools and sensors is crucial for effective physiological assessment in these models.
These advanced models hold significant promise for improving the accuracy and efficiency of heart disease research and drug discovery.

Conclusions:

Advanced 3D models utilizing human pluripotent stem cells represent a significant advancement in studying heart disease and drug responses.
Integration of tailored readouts and sensors is key to unlocking the full potential of these complex cardiac models.
These innovative approaches are poised to accelerate progress in cardiovascular research and pharmaceutical development.