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Solubilization and Bio-conjugation of Quantum Dots and Bacterial Toxicity Assays by Growth Curve and Plate Count
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Environmentally exploitable biocide/fluorescent metal marker carbon quantum dots.

Hanan B Ahmed1, Hossam E Emam2

  • 1Chemistry Department, Faculty of Science, Helwan University Ain-Helwan Cairo 11795 Egypt hananbasiony@gmail.com +201097411189.

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This study presents a green synthesis of carbon quantum dots (CQDs) from natural biopolymers. These CQDs serve as safe, fluorescent probes for detecting heavy metals and exhibit potent antimicrobial activity, offering an eco-friendly alternative to metal nanostructures.

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Area of Science:

  • Materials Science
  • Environmental Science
  • Nanotechnology

Background:

  • Metal-based nanostructures pose environmental and health risks.
  • Carbon quantum dots (CQDs) offer a safer, eco-friendly alternative due to their low toxicity and biocompatibility.
  • Developing green synthesis methods for CQDs is crucial for sustainable environmental applications.

Purpose of the Study:

  • To develop a simple, green synthesis for biocide and fluorescent marker carbon quantum dots (CQDs).
  • To evaluate the efficacy of synthesized CQDs as fluorescent probes for environmental pollutant detection (Zn2+ and Hg2+).
  • To assess the antimicrobial properties of CQDs against common bacterial and fungal pathogens.

Main Methods:

  • Synthesized CQDs using a green technique involving alkaline fragmentation and hydrothermal re-polymerization of natural biopolymers.
  • Purified CQDs via dialysis, achieving a size distribution of 1.5-6.5 nm.
  • Characterized CQDs for fluorescence properties and evaluated their performance in detecting Zn2+ and Hg2+ ions and their antimicrobial activity.

Main Results:

  • Achieved a 20-fold increase in fluorescence intensity after dialysis.
  • Successfully utilized CQDs as fluorescent markers for sensitive detection of Zn2+ and Hg2+.
  • Demonstrated significant antimicrobial potency against Bacillus cereus, Escherichia coli, and Candida albicans with a minimal inhibitory concentration of 350-450 μL mL-1.

Conclusions:

  • The presented green synthesis offers a scalable, cost-effective method for producing biocide/fluorescent CQDs.
  • These CQDs are a promising, non-toxic alternative to metal-based nanostructures for environmental monitoring and antimicrobial applications.
  • The study highlights the potential of biopolymer-derived CQDs in addressing environmental challenges safely and sustainably.