Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bistelluridopnictanes as gateways to telluradipnictiranes and a cyclic [Bi<sub>2</sub>Te<sub>2</sub>]<sup>2+</sup> dication.

Chemical science·2026
Same author

Early Outcome of Elective and Emergent Endovascular Aortic Repair with the Minos<sup>TM</sup> Abdominal Aortic Stent-Graft.

Journal of clinical medicine·2026
Same author

Engineering Nanoscale Frontiers: Valve Metal Oxide Nanostructures From Fundamentals to Multifunctional Biomedical Applications.

Chemical record (New York, N.Y.)·2026
Same author

Targeted degradation of USP7 in solid cancer cells reveals distinct effects of deubiquitinase degraders and inhibitors.

Nature communications·2026
Same author

NCOR1 and NCOR2 Exhibit Distinct Cellular and Transcriptomic Signatures in Human Abdominal Aortic Aneurysm.

Biomedicines·2026
Same author

Multinuclear Mixed-Valent Co-Oxido Complexes: Synthesis, Structure, Magnetic, and Electrochemical Properties.

Inorganic chemistry·2026

Related Experiment Video

Updated: Sep 24, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

12.2K

Lipofection with estrone-based luminophores featuring aggregation-induced emission properties.

Steffen Riebe1, Alexander Zimmermann1, Johannes Koch2

  • 1Institute for Organic Chemistry and CENIDE, University of Duisburg-Essen Universitätsstrasse 7 45141 Essen Germany jens.voskuhl@uni-due.de.

RSC Advances
|May 6, 2022
PubMed
Summary

Researchers developed novel luminophores from estrone with aggregation-induced emission (AIE) properties for efficient gene delivery. These luminescent lipoplexes enable effective transfection and tracking in HeLa and HEK 293T cells.

More Related Videos

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

13.6K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.7K

Related Experiment Videos

Last Updated: Sep 24, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

12.2K
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

13.6K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.7K

Area of Science:

  • Biochemistry
  • Materials Science
  • Nanotechnology

Background:

  • Steroid-based molecules offer unique structural properties for developing novel functional materials.
  • Aggregation-induced emission (AIE) luminophores provide enhanced signal in aggregated states, ideal for tracking and imaging applications.
  • Lipofection is a key technique for non-viral gene delivery, but efficient tracking of delivery vehicles remains a challenge.

Purpose of the Study:

  • To synthesize and characterize novel luminophores based on the steroid estrone with aggregation-induced emission (AIE) properties.
  • To develop amphiphilic molecules suitable for lipofection by incorporating cationic residues.
  • To create luminescent lipoplexes for efficient gene delivery and in situ tracking in mammalian cells.

Main Methods:

  • Synthesis of estrone-based luminophores with cationic groups.
  • Formation of self-assembled luminescent structures in aqueous media.
  • Complexation with plasmid DNA to form luminescent lipoplexes.
  • Transfection of HeLa and HEK 293T cells.
  • Tracking of lipoplexes using AIE properties.

Main Results:

  • Novel estrone-based AIE luminophores were successfully synthesized.
  • Amphiphilic molecules formed self-assembled luminescent structures.
  • Lipoplexes exhibited increased emission intensity and efficient transfection of HeLa and HEK 293T cells.
  • The AIE properties allowed for effective tracking of the lipoplexes within cells.

Conclusions:

  • Estrone-based AIE luminophores represent a promising new class of materials for biomedical applications.
  • The developed luminescent lipoplexes offer an efficient and trackable platform for gene delivery.
  • This approach facilitates real-time monitoring of gene delivery processes in cellular environments.