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Hydrogen sulfide sensing using an aurone-based fluorescent probe.

Arjun Kafle1, Shrijana Bhattarai1, Justin M Miller1,2

  • 1Molecular Bioscience Program, Middle Tennessee State University Murfreesboro TN 37132 USA justin.miller@mtsu.edu scott.handy@mtsu.edu.

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This summary is machine-generated.

Researchers developed a new, low-molecular-weight fluorescent probe for sensitive hydrogen sulfide detection. This aurone-based probe offers a fast and selective method for sensing hydrogen sulfide in biological and environmental samples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Environmental Science

Background:

  • Hydrogen sulfide (H₂S) detection is crucial for environmental monitoring and biological studies.
  • Existing sensitive fluorescent probes for H₂S often use large, complex scaffolds that are difficult to synthesize and modify.
  • There is a need for simpler, more adaptable fluorescent probes for H₂S sensing.

Purpose of the Study:

  • To design and synthesize a novel, low-molecular-weight, turn-on fluorescent probe for hydrogen sulfide detection.
  • To overcome limitations of existing probes, such as synthetic complexity, solubility, and performance in biological media.
  • To develop a sensitive, selective, and rapid method for H₂S sensing.

Main Methods:

  • Rational design and synthesis of a new aurone-based fluorescent probe, (Z)-2-(4'-azidobenzylidene)-5-fluorobenzofuran-3(2H)-one (6g).
  • Modular synthesis approach to address issues with solubility, excitation overlap, and reaction rates.
  • Utilized cationic surfactant cetyltrimethylammonium bromide (CTAB) to enhance reaction speed and fluorescence in biological media.

Main Results:

  • Successfully synthesized a low-molecular-weight aurone fluorophore probe (6g).
  • The probe demonstrated efficient and sensitive detection of hydrogen sulfide with high fluorescence enhancement.
  • Achieved a fast response time (10-30 min), good sensitivity (1 μm detection limit), and high selectivity for H₂S.
  • CTAB addition improved reaction kinetics and fluorescence intensity in biologically relevant conditions.

Conclusions:

  • The developed aurone-based fluorescent probe offers a significant advancement for hydrogen sulfide detection.
  • Its low molecular weight, modular synthesis, and improved performance in biological media make it highly applicable.
  • This probe provides a sensitive, selective, and rapid tool for both environmental and biological H₂S sensing applications.