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Updated: Sep 24, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Parallel G-quadruplex-mediated protein dimerization and activation
Tuom Tinh Thi Truong1, Cuong Cao2, Dung Thanh Dang1,3
1University of Science, Vietnam National University Ho Chi Minh City Vietnam dung.dthanh@ou.edu.vn.
Abstract:
We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed in the presence of parallel G4, which indicated that parallel G4 can induce dimerization and activation of proteins. This novel approach holds a great promise for studying G4-targeting functional dimeric proteins in celllular biology.
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