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Related Experiment Video

Updated: Sep 24, 2025

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Open source fraction collector/MALDI spotter for proteomics.

Scott B Ficarro1, William Max Alexander1, Isidoro Tavares1

  • 1Department of Cancer Biology, Blais Proteomics Center, Dana-Farber Cancer Institute, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 360 Longwood Avenue, LC 2208, Boston, MA 02215-5450, USA.

Hardwarex
|May 6, 2022
PubMed
Summary
This summary is machine-generated.

We developed an affordable, open-source system for thermostatted peptide fraction collection, enhancing mass spectrometry analysis of complex proteomes. This customizable solution simplifies sample preparation for techniques like MALDI-MS.

Keywords:
Fraction collectionHPLC, high pressure liquid chromatographyLC, liquid chromatographyLC-MALDILC-MS, Liquid Chromatography/Mass spectrometryMALDI-MS, matrix-assisted laser desorption ionization mass spectrometryMS, mass spectrometer/mass spectrometryOEM, Original Equipment ManufacturerOpen sourceProteomicsPython

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry is crucial for analyzing complex proteomes.
  • High-performance peptide fraction collection is essential for sensitive mass spectrometry.
  • Commercial systems for peptide fractionation are often expensive and lack customization.

Purpose of the Study:

  • To present a cost-effective, open-source hardware and software solution for thermostatted peptide fraction collection.
  • To enable high-performance sample preparation for mass spectrometry experiments.
  • To provide a customizable platform for researchers working with complex proteomes.

Main Methods:

  • Developed a complete open-source hardware system for peptide fraction collection.
  • Implemented a Python-based software for instrument control and customization.
  • Utilized readily available retail components for instrument assembly.
  • Integrated thermostatted fraction collection for enhanced peptide stability.

Main Results:

  • The system offers high-performance thermostatted peptide fraction collection.
  • The instrument is assembled from affordable, readily available parts.
  • The open-source software allows for rapid customization.
  • Demonstrated applications include automated deposition for MALDI-MS and fraction collection for nanoflow HPLC.

Conclusions:

  • This open-source solution significantly reduces the cost of high-performance peptide fractionation systems.
  • The customizable and accessible platform supports advanced mass spectrometry applications.
  • The system facilitates complex proteome analysis through improved sample preparation.