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Updated: Sep 24, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Simultaneous detection of multiple pathogens with the TaqMan Array Card.

Rachael Lappan1, Thanavit Jirapanjawat1, Deborah A Williamson2

  • 1Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Methodsx
|May 6, 2022
PubMed
Summary
This summary is machine-generated.

The TaqMan Array Card (TAC) enables simultaneous detection of multiple pathogens, overcoming limitations of standard quantitative polymerase chain reaction (qPCR). This cost-effective method is ideal for large-scale pathogen screening across diverse sample types.

Keywords:
EnteropathogenOne healthSurveillanceqPCR

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Disease Diagnostics

Background:

  • Quantitative polymerase chain reaction (qPCR) is a sensitive and specific method for pathogen detection but is labor-intensive for multiplexed screening.
  • Multiplexing qPCR is limited in sample throughput when analyzing numerous pathogens simultaneously.
  • The TaqMan Array Card (TAC) offers a scalable solution for high-throughput pathogen detection.

Purpose of the Study:

  • To present a protocol for utilizing the TaqMan Array Card (TAC) for simultaneous detection of multiple pathogens.
  • To expand the application of TAC beyond gene expression to pathogen detection in various sample types.
  • To provide guidelines for data processing and interpretation for accurate results.

Main Methods:

  • Utilized the TaqMan Array Card (TAC) platform for multiplexed pathogen detection.
  • Developed a laboratory setup protocol for TAC implementation.
  • Established data processing and interpretation guidelines, including video assistance.

Main Results:

  • Demonstrated the application of TAC for monitoring a wide range of gastrointestinal pathogens (viral, bacterial, protist, helminth).
  • Successfully adapted TAC for pathogen detection in human, animal, and environmental samples.
  • Circumvented issues with automated TAC outputs through developed processing and interpretation guidelines.

Conclusions:

  • The TaqMan Array Card (TAC) is a cost-effective and accurate technique for simultaneous detection of multiple pathogens.
  • TAC significantly enhances the capacity for large-scale pathogen screening across diverse sample matrices.
  • This adaptable technique is valuable for organism detection in any field requiring quality nucleic acid analysis.