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Updated: Sep 24, 2025

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
Fluorescence detection of fluorine ions in biological fluids based on aggregation-induced emission
Yanming Miao1, Wenli Yang1, Jinzhi Lv1
1Shanxi Normal University Linfen 041004 PR China mym8207@126.com +86-357-2051243 +86-357-2051249.
Abstract:
Traditional chemical and biological sensors developed through aggregation-induced emission (AIE) are mainly based on "Turning on" pattern of fluorescence enhancement, which often has poor selectivity and can be easily interfered with by other substances. On this basis, an AIE-based tetraphenyl ethylene (TPE) derivative (TPE-COOH) was prepared in this study and aggregated by adding Al3+, so as to form the TPE-COOH/Al3+ polymer. TPE-COOH fluorescence was enhanced through AIE principle, thus realizing the "Turning on" state. F- could bind to Al3+ after the addition of F- ions which would result in the decomposition of TPE-COOH/Al3+ aggregate, dissolved state of TPE-COOH and gradual reduction of fluorescence intensity of the system, thus realizing "Turning off" state. Moreover, F- ions in biological fluid were analyzed and detected through such AIE-based "Turning on-off" pattern. The linear range of this method for F- detection was 3-12 μM and the detection limit was 0.9 μM.
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