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Updated: Sep 24, 2025
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
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A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR-Cas9 modified catalytic
Yang Liu1, Shihong Li1, Likun Zhang1
1Department of Clinical Laboratory, The Third Affiliated Hospital of Jinzhou Medical University Jinzhou Liaoning Province 121000 China jykly43@163.com.
RSC Advances
|May 6, 2022
Abstract:
We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection and in situ miR-21 imaging.
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