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Updated: Sep 24, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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High-resolution 3D Fourier ptychographic reconstruction using a hemispherical illumination source with

Minglu Sun1,2,3, Lina Shao4,3, Jinrui Zhang4

  • 1State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.

Biomedical Optics Express
|May 6, 2022
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Summary

This study enhances Fourier ptychography (FP) for real-time 3D imaging. A novel hemispherical illumination and coded patterns achieve high-resolution 3D refractive index reconstruction at over 1 Hz.

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Area of Science:

  • Optical imaging
  • Microscopy
  • Quantitative phase imaging

Background:

  • Fourier ptychography (FP) enables high-resolution 2D quantitative reconstruction.
  • FP platforms can be extended for 3D refractive index (RI) reconstruction.
  • Traditional FP faces limitations in illumination numerical aperture (NA) and intensity attenuation.

Purpose of the Study:

  • To improve illumination NA and reduce intensity attenuation in FP.
  • To achieve high-resolution 3D RI reconstruction.
  • To enable real-time 3D imaging of live samples.

Main Methods:

  • Implemented a hemispherical lighting structure with a new LED arrangement based on 3D Fourier diffraction theory.
  • Achieved high NA illumination (0.98 NA) using 187 LEDs.
  • Employed a multiplexed-coded illumination strategy with a radial-coded pattern for faster acquisition.

Main Results:

  • Attained lateral and axial half-pitch resolutions of ~174 nm and ~524 nm, respectively, with a 40×/0.6NA objective.
  • Demonstrated acquisition speeds exceeding 1 Hz with the radial-coded pattern.
  • Showcased results comparable to sequential scanning methods.

Conclusions:

  • The developed FP system significantly enhances 3D RI reconstruction capabilities.
  • The hemispherical illumination and coded patterns enable high-resolution, real-time 3D imaging.
  • This technique holds promise for in vitro live sample observation.