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Updated: Sep 24, 2025

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
Published on: September 22, 2020
Preparation of cationic proteoliposomes using cell-free membrane protein synthesis: the chaperoning effect of
Mitsuru Ando1, Yoshihiro Sasaki1, Kazunari Akiyoshi1
1Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Katsura, Nishikyo-ku Kyoto 615-8510 Japan akiyoshi@bio.polym.kyoto-u.ac.jp +81-75-383-2590 +81-75-383-2589.
Abstract:
Membrane protein reconstituted cationic liposomes are constructed using cell-free membrane protein synthesis in the presence of cationic liposomes. The chaperon effect of cationic liposomal membrane assists in folding the functional conformation of membrane protein. This preparation method enables the provision of the usage of proteoliposomes for drug delivery.

