Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Drug Distribution: Tissue Binding

Drug Distribution: Tissue Binding

Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engrailed, a target of the miR-92 family, attenuates Tau accumulation and associated memory deficits in Drosophila.

Biochimica et biophysica acta. Molecular cell research·2026

Same author

Design of an ultra-compact and high-performance antenna for private 5G applications.

Scientific reports·2026

Same author

High-yield cell-derived extracellular matrix bioink via macromolecular crowding for versatile 3D bioprinting.

Materials today. Bio·2026

Same author

Dysregulation of xenobiotic metabolism and mitochondrial dysfunction exacerbate acetaminophen-induced hepatotoxicity in human antigen R-deficient male mice.

Toxicology·2026

Same author

High gain and slant dual-polarized antenna for private 5G railway base stations.

Scientific reports·2026

Same author

Dynamic monitoring of bone homeostasis by dual fluorescent imaging of osteoblast and osteoclast activity.

Acta biomaterialia·2026

Search research articles

Related Experiment Video

Updated: Sep 24, 2025

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Published on: February 4, 2017

Bone-targeting carbon dots: effect of nitrogen-doping on binding affinity.

Kyung Kwan Lee^1,2, Jae-Geun Lee^3,4, Chul Soon Park⁵

¹Hazards Monitoring BNT Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 34141 Republic of Korea.

|May 6, 2022

Summary

Novel fluorescent carbon dots (CDs) utilizing alendronate show enhanced bone targeting for imaging and drug delivery. These alendronate-based CDs (Alen-CDs) demonstrate strong binding to bone components and sustained fluorescence in vivo.

More Related Videos

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

Published on: May 13, 2013

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

Related Experiment Videos

Last Updated: Sep 24, 2025

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Published on: February 4, 2017

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

Published on: May 13, 2013

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

Area of Science:

Biomaterials Science
Nanotechnology
Medical Imaging

Background:

Bone imaging and targeted therapies are crucial for treating bone diseases.
Developing novel fluorescent probes with high bone affinity is an ongoing challenge.
Alendronate, a bisphosphonate, is known for its bone-targeting properties.

Purpose of the Study:

To synthesize and characterize novel fluorescent carbon dots (CDs) for enhanced bone imaging and targeted delivery.
To investigate the bone-targeting capabilities of alendronate-based CDs (Alen-CDs) compared to nitrogen-doped CDs.
To evaluate the in vitro, ex vivo, and in vivo performance of Alen-CDs for bone applications.

Main Methods:

Facile hydrothermal synthesis of alendronate-based carbon dots (Alen-CDs).
Assessment of binding affinity to calcium-deficient hydroxyapatite (CDHA) and bone tissues (rat femur, zebrafish).
In vivo studies in zebrafish larvae and ex vivo analysis of rat femur tissues.

Main Results:

Alen-CDs exhibited strong binding to CDHA and bone structures due to surface bisphosphonate groups.
In vivo studies showed effective accumulation and sustained skeletal fluorescence (7 days post-injection) in zebrafish larvae.
Alen-CDs demonstrated superior toleration and bone targeting compared to nitrogen-doped counterparts and Alizarin Red S.

Conclusions:

Alen-CDs are effective fluorescent probes for bone imaging with high bone affinity.
The bisphosphonate group on CDs is key for direct bone targeting and sustained fluorescence.
Alen-CDs show significant potential for targeted drug delivery to bone and treating bone diseases like osteoporosis.