Research progress in the development of organic small molecule fluorescent probes for detecting H₂O₂

Hydrogen peroxide (H₂O₂), as an important signaling molecule during biological metabolism, is a key member of the reactive oxygen species (ROS) family. The excess of H₂O₂ will lead to oxidative stress, which is a crucial factor in the production of various ROS-related diseases. In order to study the diverse biological roles of H₂O₂ in cells and animal tissues, many methods have been developed to detect H₂O₂. Recently, fluorescence imaging has attracted more and more attention because of its high sensitivity, simple operation, experimental feasibility, and real-time online monitoring. Based on the response group, this study will review the research progress on hydrogen peroxide and summarizes the mechanisms, actualities and prospects of fluorescent probes for H₂O₂.