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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission
Hui Zhang1,2, Lizhen Xu1, Wenxiu Li3
1College of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Abstract:
A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.
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