Effect of demycarosylturimycin on ribosomal peptidyltransferase from Escherichia coli

The effect of demycarosylturimycin H on bacterial growth, polypeptide synthesis and peptidyltransferase was studied. The effects are compared with those of intact turimycins acylated or deacylated in 4''-position of mycarose. The removal of the acyl group in 4''-position of mycarose leads to a total loss of inhibition of acceptor substrate binding while the removal of acyl mycarose moiety changes the pattern of inhibition of polypeptide synthesis.