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Updated: Sep 24, 2025
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
EVALUATION OF NLRP3 INFLAMMASOME PROTEIN EXPRESSION IN ULCERATIVE COLITIS
1MIDDLE TECHNICAL UNIVERSITY, BAGHDAD, IRAQ.
Objective:
The aim: This study aimed to evaluate the NLRP3 immunoreactivity in ulcerative colitis in various clinical presentations.
Patients And Methods:
Materials and methods: The retrospective study involved 80 formalin fixed paraffin embedded tissue blocks. These were divided into 50 samples of ulcerative colitis and 30 with normal colonoscopy findings. NLRP3 protein expression patterns were evaluated in various cellular components in tissue sections using immunohistochemistry method.
Results:
Results: NLRP3 inflammasome was significantly expressed with higher percentage among ulcerative colitis tissue sections compared with normal tissue sections. Intense staining was observed in Paneth cells at the base of crypts, inflammatory cells infiltrated between glands and near the base of crypts. Variable intensities of NLRP3 staining were observed in surface epithelial cells and glandular epithelium. Statically higher percentage of expression was found among active disease and patients with extra-intestinal complications.
Conclusion:
Conclusions: The NLRP3 protein expression pattern was upregulated among various cellular compartments among ulcerative colitis and correlated with disease activity.
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