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Fungicidal activity of murine inflammatory polymorphonuclear neutrophils: comparison with murine peripheral blood PMN
Abstract:
A characteristic of inflammation is the influx of polymorphonuclear neutrophils (PMN) from peripheral blood to the inflammatory reaction. We report on metabolic alterations and alterations in fungicidal activity in PMN elicited intraperitoneally with different inflammatory agents. The fungicidal activity of murine peripheral blood PMN (PB-PMN) against phagocytosable Candida albicans and nonphagocytosable Blastomyces dermatitidis was compared to that of murine inflammatory PMN. PMN elicited with sodium caseinate exhibited enhanced killing of B. dermatitidis (93 +/- 3%) compared to PB-PMN (38 +/- 11.7%). In contrast, thioglycollate medium elicited PMN had significantly less ability to kill B. dermatitidis (3 +/- 5%) than PB-PMN. Incubation of caseinate PMN with thioglycollate medium for 1 h significantly reduced their ability to kill B. dermatitidis (95% vs 25%). This effect was not due to cytotoxicity of thioglycollate medium for PMN. Candidacidal activity of inflammatory PMN (caseinate or proteose peptone-elicited) was not significantly greater than that of peripheral blood PMN. However, inflammatory PMN had significantly greater candidacidal activity than thioglycollate-elicited PMN.
Insights
Inflammatory agents alter polymorphonuclear neutrophil (PMN) fungicidal activity. Caseinate-elicited PMN effectively kill Blastomyces dermatitidis, while thioglycollate-elicited PMN show reduced activity against this fungus.
Area of Science:
- Immunology
- Microbiology
Background:
- Inflammation involves polymorphonuclear neutrophil (PMN) migration.
- PMN fungicidal activity is crucial for host defense.
Purpose of the Study:
- To investigate metabolic and fungicidal alterations in PMN elicited by different inflammatory agents.
- To compare the fungicidal activity of peripheral blood PMN (PB-PMN) with elicited PMN against Candida albicans and Blastomyces dermatitidis.
Main Methods:
- Murine PMN were elicited intraperitoneally using sodium caseinate or thioglycollate medium.
- Fungicidal activity assays were performed against Candida albicans and Blastomyces dermatitidis.
- Caseinate-elicited PMN were incubated with thioglycollate medium to assess its effect on fungicidal activity.
Main Results:
- Sodium caseinate-elicited PMN showed significantly enhanced killing of Blastomyces dermatitidis compared to PB-PMN.
- Thioglycollate medium-elicited PMN exhibited significantly reduced killing of Blastomyces dermatitidis.
- Incubation with thioglycollate medium impaired the fungicidal capacity of caseinate-elicited PMN against B. dermatitidis.
- Elicited PMN (caseinate or proteose peptone) did not show enhanced candidacidal activity compared to PB-PMN, but thioglycollate-elicited PMN were less effective.
Conclusions:
- The inflammatory agent used to elicit PMN significantly impacts their fungicidal activity, particularly against non-phagocytosable fungi like B. dermatitidis.
- Thioglycollate medium can suppress the fungicidal capabilities of PMN.
- PMN fungicidal activity is context-dependent and influenced by the inflammatory microenvironment.