Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Differential gene expression profiles of DNA repair genes in esophageal cancer cells after X-ray irradiation.

Chinese journal of cancer·2010

Same author

Identification of differentially expressed genes related to radioresistance of human esophageal cancer cells.

Chinese journal of cancer·2010

Same author

[Rapid identification of cortex dictamni pieces and its counterfeit alangium Chinense by spectral imaging method].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2010

Same author

Cleavage and reorganization of Zr-C/Si-C bonds leading to Zr/Si-N organometallic and heterocyclic compounds.

Journal of the American Chemical Society·2010

Same author

Abl tyrosine kinase phosphorylates nonmuscle Myosin light chain kinase to regulate endothelial barrier function.

Molecular biology of the cell·2010

Same author

[Modified silica gel for absorption of ammonia].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2010

Search research articles

Related Experiment Video

Updated: Sep 24, 2025

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Published on: April 1, 2020

[An optical parameter imaging system with profile information fusion].

Tongxin Li^1,2, Yeqing Dong^1,2, Ming Liu³

¹Tianjin Anding Hospital, Tianjin 300222, P. R. China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi

|May 6, 2022

Summary

This summary is machine-generated.

This study introduces an advanced imaging system to accurately measure optical parameters in complex biological tissues. The novel method corrects for surface variations, significantly improving measurement precision for diverse clinical applications.

Keywords:

Complex profiles Laws of illumination Spatial frequency domain imaging The optical parameters

More Related Videos

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Sep 24, 2025

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Published on: April 1, 2020

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

Biomedical Optics
Optical Imaging
Biophysical Measurement

Background:

Accurate optical parameter measurement in biological tissues is crucial for diagnostics and research.
Complex tissue topography presents a significant challenge for existing optical imaging techniques.
Current methods often struggle with variations in surface height and angle, leading to inaccuracies.

Purpose of the Study:

To develop and validate an imaging system for precise optical parameter retrieval from biological tissues with complex profiles.
To address the limitations of current optical imaging by incorporating profile correction.
To demonstrate the clinical applicability and versatility of the proposed imaging approach.

Main Methods:

Utilized Fourier transformation profilometry to capture detailed surface topography of biological tissues.
Applied laws of illumination to correct for variations in incident light intensity across the tissue surface.
Integrated spatial frequency domain imaging with profile correction for accurate optical parameter determination.

Main Results:

The proposed system accurately and rapidly obtains both profile information and optical parameters.
Profile correction significantly reduced relative errors in optical parameter measurements (e.g., from >46% to <7% for height variations).
Successful imaging of a face-like phantom and a human prefrontal lobe demonstrated practical utility.

Conclusions:

The developed imaging system effectively measures optical parameters in complex biological tissues.
The profile correction method enhances the accuracy of optical imaging and can be integrated with existing technologies.
This approach holds significant clinical application value for non-invasive tissue characterization.