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Salmonella typhimurium mutants lacking protease II.

C Heiman, C G Miller

    Journal of Bacteriology
    |August 1, 1978
    PubMed
    Summary
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    Researchers isolated Salmonella typhimurium mutants lacking protease II, an enzyme crucial for protein degradation. These findings are vital for understanding bacterial protein turnover and cellular processes.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Enzymology

    Background:

    • Salmonella typhimurium possesses various proteases, including protease II, an endoprotease with trypsin-like specificity.
    • The precise roles of specific proteases in bacterial protein degradation and turnover are not fully elucidated.

    Purpose of the Study:

    • To isolate and characterize mutants of Salmonella typhimurium deficient in protease II.
    • To investigate the genetic locus of protease II and its relationship with other genes.
    • To assess the impact of lacking protease II on bacterial growth, protein degradation, and turnover.

    Main Methods:

    • Isolation of protease II-deficient mutants using a chromogenic substrate (N-methyl-N-p-toluenesulfonyl-L-lysine beta-naphthyl ester).
    • Genetic mapping of mutations to the tlp locus using phage P1-mediated cotransduction with the tre locus.

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  • Construction and phenotypic analysis of double mutants lacking both protease I and protease II.

    • Main Results:

    • Successfully isolated Salmonella typhimurium mutants lacking protease II.
    • Identified and mapped the mutations to the tlp locus, showing cotransduction with the tre locus.
    • Demonstrated that double mutants lacking both protease I and protease II exhibit normal growth.
    • Confirmed that these double mutants maintain wild-type rates of abnormal protein degradation and protein turnover during carbon starvation.

    Conclusions:

    • Protease II is not essential for Salmonella typhimurium growth or viability.
    • The absence of protease II, even in combination with protease I deficiency, does not impair essential protein degradation and turnover processes.
    • The tlp locus is a distinct genetic locus involved in protease II production.