Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008
Same journal

QbD-guided development and in silico, in vitro, ex vivo, and in vivo dermatokinetic evaluation of a fluvastatin-loaded transferosomal hydrogel for the management of melanoma.

Biomaterials advances·2026
Same journal

Hydrothermally fabricated chitosan-alginate polyelectrolyte complex-based hemostatic patches with shape recovery.

Biomaterials advances·2026
Same journal

A multifunctional composite film comprising a citric acid-crosslinked polysaccharide matrix loaded with nutmeg essential oil nanoemulsion: suitable for facilitating active wound healing.

Biomaterials advances·2026
Same journal

Graphene oxide functionalized with polyamidoamine dendrimer as a drug delivery system for human breast cells.

Biomaterials advances·2026
Same journal

Drug-loaded biomorphic scaffolds for tissue regeneration and targeted antitumor therapy in bone metastasis models.

Biomaterials advances·2026
Same journal

Adding beta-tricalcium phosphate ceramic to additive manufactured 3D porous Ti6Al4V scaffolds enhances osteogenic activity of human mesenchymal stromal cells in vitro.

Biomaterials advances·2026
See all related articles

Related Experiment Video

Updated: Sep 24, 2025

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research
08:06

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research

Published on: May 21, 2008

8.4K

Customized construction of microscale multi-component biostructures for cellular applications.

Zhixing Ge1, Haibo Yu2, Wenguang Yang3

  • 1State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China; Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Biomaterials Advances
|May 6, 2022
PubMed
Summary
This summary is machine-generated.

A novel digital micromirror device-based optical projection lithography (DOPL) system enables high-resolution 3D microfabrication for advanced cell applications. This technology facilitates rapid cell spheroid formation, selective cell adhesion, and biomimetic model construction for drug screening and tissue engineering.

Keywords:
Cellular applicationDigital micromirror device (DMD)MicroscaleMulti-component biofabrication

More Related Videos

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
09:37

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Published on: May 12, 2008

11.9K
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

6.7K

Related Experiment Videos

Last Updated: Sep 24, 2025

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research
08:06

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research

Published on: May 21, 2008

8.4K
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
09:37

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Published on: May 12, 2008

11.9K
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

6.7K

Area of Science:

  • Biomaterials Engineering
  • Biomedical Engineering
  • Cellular Engineering

Background:

  • In vitro biofabrication methods face limitations in resolution, component range, and precise 3D cell deposition.
  • Existing techniques struggle to create complex microstructures with controlled cellular organization.

Purpose of the Study:

  • To introduce a digital micromirror device-based optical projection lithography (DOPL) system for high-resolution 3D microfabrication.
  • To explore the system's capabilities in creating multi-component microstructures and investigate cellular responses.

Main Methods:

  • Utilized digital micromirror device-based optical projection lithography (DOPL) for microfabrication.
  • Employed polyethylene glycol dimethacrylate (PEGDMA) and gelatin methacryloyl (GelMA) hydrogels for microstructure construction.
  • Investigated printability, mechanical properties, and cell behavior within fabricated structures.

Main Results:

  • Achieved resolutions of tens of microns, enabling rapid fabrication of micropit arrays in PEGDMA hydrogels.
  • Demonstrated rapid, high-throughput formation of uniform cell spheroids in micropits.
  • Successfully constructed multi-component microstructures with selective cell adhesion at heterogeneous interfaces.
  • Fabricated a biomimetic peritoneal model.

Conclusions:

  • The DOPL system offers high resolution and versatility for creating complex 3D microstructures.
  • The system effectively supports rapid cell spheroid formation and controlled cellular organization.
  • DOPL shows significant potential for applications in cell behavior research, drug screening, and tissue engineering.