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Updated: Sep 24, 2025
12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
5.2K
Cyanobacterial Genome Sequencing, Annotation, and Bioinformatics
Jonna Teikari1,2,3, Martin Baunach4, Elke Dittmann4
1Environmental Soil Science, Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland. jonna.teikari@helsinki.fi.
Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2022
Summary
This study presents a genome sequencing pipeline for cyanobacteria, a globally important bacterial phylum. The pipeline aids in understanding cyanobacterial diversity and bioactive compound discovery.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Cyanobacteria are a globally significant bacterial phylum with diverse bioactive products and ecological roles.
- Advancements in sequencing technologies have increased the availability of cyanobacterial genomes.
- Comprehensive understanding of the molecular basis of cyanobacterial diversity requires further investigation.
Purpose of the Study:
- To introduce a standardized pipeline for cyanobacterial genome sequencing.
- To facilitate the completion of whole cyanobacterial genomes.
- To provide guidance for genome mining and analysis.
Main Methods:
- DNA extraction from cyanobacterial cultures.
- Hybrid genome sequencing.
- Genome assembly and annotation.
- Introduction to genome mining tools.
Main Results:
- A comprehensive pipeline for cyanobacterial genome sequencing is presented.
- The pipeline covers DNA extraction, sequencing, assembly, and annotation.
- Genome mining tools and a case study are briefly discussed.
Conclusions:
- The provided pipeline offers a foundational framework for cyanobacterial genome projects.
- The pipeline can be adapted to specific laboratory needs.
- This work supports further exploration of cyanobacterial molecular diversity and applications.
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