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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

466
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
466
CRISPR01:59

CRISPR

53.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.1K

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Related Experiment Video

Updated: Sep 24, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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Optimized Tools and Methods for Methanotroph Genome Editing.

Sreemoye Nath1,2, Jessica M Henard2, Calvin A Henard3,4

  • 1Department of Biological Sciences, University of North Texas, Denton, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2022
PubMed
Summary
This summary is machine-generated.

Methanotrophs, microbes that consume methane, are key for bioconversion and atmospheric methane cycling. New genetic tools now enable deeper understanding of their physiology and metabolic engineering for valuable products.

Keywords:
BiocatalysisBiogasBroad host-range plasmidCRISPRConjugationGenetic engineeringGenome editingMetabolic engineeringMethanotrophNatural gas

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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Area of Science:

  • Microbiology
  • Biotechnology
  • Environmental Science

Background:

  • Methanotrophs utilize methane (CH4) for bioconversion and are crucial for atmospheric CH4 cycling.
  • Existing knowledge of methanotrophs is limited by a lack of efficient molecular tools for studying gene-function relationships.

Purpose of the Study:

  • To introduce novel genetic tools and optimized genome editing methods for methanotrophs.
  • To facilitate metabolic engineering and explore physiological mechanisms in these bacteria.

Main Methods:

  • Development of new genetic tools.
  • Optimization of genome editing techniques for methanotrophs.

Main Results:

  • Enabling of advanced metabolic engineering in methanotrophs.
  • Facilitating the investigation of methanotroph physiology and responses to stimuli.

Conclusions:

  • The described tools significantly advance the study and engineering of methanotrophs.
  • These advancements hold potential for bioconversion applications and understanding biogeochemical cycles.