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Updated: Sep 24, 2025
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Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery
Published on: July 18, 2019
8.6K
Genetically engineered antibodies and their application to brain delivery.
Sherie L Morrison1, Seung-Uon Shin
1Department of Microbiology and Molecular Genetics, 507 The Molecular Biology Institute, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA.
Advanced Drug Delivery Reviews
|May 7, 2022
Summary
Genetic engineering enables novel antibody production, offering alternatives to animal immunization and overcoming limitations of traditional monoclonal antibodies for therapeutic applications.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Traditional antibody production methods face limitations.
- Genetic engineering offers advanced solutions for antibody development.
Purpose of the Study:
- To explore genetic engineering techniques for novel antibody production.
- To evaluate modified antibodies for therapeutic potential and new applications.
Main Methods:
- Utilizing bacteriophage combinatorial libraries for antigen-binding specificities.
- Employing transfectomas for genetically engineered antibody gene expression.
- Creating modified antibodies: chimeric, humanized, catalytic, bifunctional, and fragment forms.
Main Results:
- Novel antibodies with retained or enhanced functions were produced.
- Modified antibodies demonstrated potential in immunotherapy, with challenges in immunogenicity.
- Antibody fusion proteins showed increased brain parenchyma uptake.
Conclusions:
- Genetic engineering provides versatile strategies for antibody development.
- Modified antibodies hold promise for diverse therapeutic and diagnostic applications.
- Further research into effector functions and immunogenicity is crucial for optimizing therapeutic antibodies.
