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Related Experiment Videos

An induced synovitis disease model in ponies.

E C Firth, T Wensing, F Seuren

    The Cornell Veterinarian
    |April 1, 1987
    PubMed
    Summary

    Lipopolysaccharide (LPS) injections in pony joints mimic acute bacterial infections, showing rapid onset and resolution of clinical signs. This model is valuable for studying infectious joint disease mechanisms.

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    Preventive veterinary medicine·2012

    Area of Science:

    • Veterinary Medicine
    • Immunology
    • Equine Science

    Background:

    • Acute bacterial infection is a significant cause of lameness and joint disease in horses.
    • Understanding the early inflammatory response in joints is crucial for effective treatment.
    • Lipopolysaccharide (LPS) from E. coli is a potent bacterial component that elicits inflammatory responses.

    Purpose of the Study:

    • To investigate the effects of intra-articular E. coli lipopolysaccharide (LPS) injection in ponies.
    • To evaluate the clinical, hematological, and synovial fluid changes induced by LPS.
    • To determine the utility of this model for studying infectious joint disease.

    Main Methods:

    • Intra-articular injection of small amounts of E. coli LPS into the intercarpal joint of 5 ponies.
    • Monitoring of clinical signs including fever, depression, appetite, vital signs, and lameness.
    • Collection and analysis of blood and synovial fluid for hematological parameters, protein, leucocytes, and alkaline phosphatase.
    • Assessment of mucin precipitation in synovial fluid.

    Main Results:

    • LPS induced rapid onset (1-2 hours) and short-lived (36 hours) signs analogous to acute bacterial arthritis.
    • Fever peaked at 5-7 hours; clinical signs included depression, anorexia, increased vital signs, and lameness.
    • Hematological changes included neutrophilic leucocytosis and altered serum copper, iron, and zinc.
    • Synovial fluid analysis showed increased total protein, leucocytes, and alkaline phosphatase within 2 hours.
    • Synovial fluid abnormalities persisted longer than clinical and hematological changes.

    Conclusions:

    • Intra-articular LPS injection in ponies provides a reproducible model for acute infectious joint disease.
    • The model allows for early study of inflammatory processes and resolution dynamics.
    • This experimental approach is useful for investigating specific aspects of infectious arthritis in equine joints.

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