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Measurement of postmortem outflow facility using iPerfusion.

Michael Madekurozwa1, Ester Reina-Torres1, Darryl R Overby1

  • 1Dept. of Bioengineering, Imperial College London, London, SW7 2AZ, UK.

Experimental Eye Research
|May 7, 2022
PubMed
Summary
This summary is machine-generated.

This study shows that postmortem mouse eyes can accurately measure aqueous humor outflow facility, crucial for glaucoma research. Controlling humidity is key to avoid evaporation artifacts and ensure reliable results for intraocular pressure studies.

Keywords:
Mouse modelsOutflow facilityPerfusionPostmortem effects

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Area of Science:

  • Ophthalmology
  • Physiology
  • Biomedical Engineering

Background:

  • Glaucoma, a leading cause of vision loss, is characterized by elevated intraocular pressure (IOP).
  • Aqueous humor outflow facility is a critical parameter for understanding IOP regulation and developing glaucoma treatments.
  • Mouse eyes are a valuable model for studying human aqueous humor dynamics due to anatomical similarities and pharmacological responses.

Purpose of the Study:

  • To investigate the utility of the postmortem in situ mouse eye model for measuring aqueous humor outflow facility using the iPerfusion system.
  • To assess pressure-independent flow, the impact of evaporation, and the pressure dependence of outflow facility in postmortem eyes.
  • To compare the postmortem in situ model with the established ex vivo model for IOP research.

Main Methods:

  • Utilized the iPerfusion system for perfusing postmortem mouse eyes within a cadaver.
  • Investigated pressure-independent flow and outflow facility across a range of pressures.
  • Employed a humidity chamber to evaluate the effect of environmental humidity on measured flow rates.
  • Assessed the influence of postmortem delay on outflow facility measurements.

Main Results:

  • Demonstrated negligible pressure-independent flow in postmortem eyes, contrary to prior assumptions.
  • Identified significant evaporative artifacts at room humidity, which were eliminated in a high humidity (>85%) environment.
  • Reported average postmortem outflow facility of ~4 nl/min/mmHg, comparable to ex vivo values, with reduced intra-animal variability.
  • Observed reduced pressure-dependence of outflow facility in the postmortem model, particularly when cannulation occurred >40 minutes post-euthanasia.

Conclusions:

  • The postmortem in situ mouse eye model, when properly controlled for humidity, offers a viable alternative to ex vivo preparations for measuring aqueous humor outflow facility.
  • This model provides reduced variability and pressure-dependence in outflow facility measurements, enhancing its utility for glaucoma research.
  • Careful control of experimental conditions, especially humidity, is essential for accurate and reproducible results in postmortem eye perfusion studies.