Immobilization of cadmium by mercapto-functionalized palygorskite under stimulated acid rain: Stability performance...

The interaction of cadmium (Cd) pollution and acid rain stress has seriously threatened soil ecosystem and human health. However, there are still few effective amendments for the in-situ remediation in the Cd-contaminated acidified soil. In this study, the performance and mechanisms of palygorskite (PAL) and mercapto-functionalized PAL (MPAL) on Cd immobilization were investigated, and the stability as well as effects on soil micro-ecology under stimulated acid rain were also explored. Results showed that MPAL could react with Cd to form stable Cd-sulfhydryl and Cd-O complexes. The reduction of bioavailable Cd by MPAL was 121.19-164.86% higher than that by PAL. Notably, the Cd immobilization by MPAL remained stable within 90 days in which the concentrations of HOAc-extractable Cd were reduced by 18.28-25.12%, while the reducible and residual fractions were increased by 9.26-18.53% and 54.16%-479.01%, respectively. The sequential acid rain leaching demonstrated that soil after MPAL treatments had a strong H⁺ resistance, and the immobilized Cd showed prominent stability. In addition, activities of acid phosphatase, catalase and invertase in MPAL treated soil were significantly enhanced by 34.60%, 22.09% and 48.87%, respectively. After MPAL application, bacterial diversity was further improved with diversified sulfur metabolism biomarkers. The decreased abundance of Cd resistance genes including cadA, cadC, czcA, czcB, czcR and zipA also indicated that soil micro-ecology was improved by MPAL. These results showed that MPAL was an effective and eco-friendly amendment for the immobilization of Cd in contaminated soil.