CD9 expression in porcine blood CD4⁺ T cells delineates two subsets with phenotypic characteristics of central and...

In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4⁺ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4⁺ T cell development. CD4⁺ 2E3⁺ CD8α^- CD9^- cells are the least differentiated population of naïve cells, whereas the CD4⁺ 2E3^- CD8α⁺CD9⁺ and CD4⁺ 2E3^- CD8α⁺ CD9^- cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9⁺ phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4⁺ T cell heterogeneity in swine.