One-step sodium bisulfate hydrolysis for efficient production of xylooligosaccharides from poplar

Acid salts have been shown to catalyze xylan hydrolysis selectively and efficiently for xylooligosaccharides (XOS) production while using acid salts that are non-toxic and available as feed additives can avoid separation from resulting XOS-rich hydrolysates. There is no report on XOS production with sodium bisulfate (NaHSO₄) hydrolysis, of significance is that NaHSO₄ as feed additive does not need to be separated. In this work, NaHSO₄ hydrolysis was firstly employed to produce XOS from poplar. XOS yield of 42.7% was reached under optimal conditions of 0.04 mol/L NaHSO₄, 170 °C and 60 min. After hydrogen peroxide/acetic acid and sodium hydroxide treatments of NaHSO₄-pretreated poplar, high yields of glucose (92.0%) and xylose (91.3%) were obtained at a low cellulase dose of 5 FPU/g dry mass. NaHSO₄ hydrolysis was a novel strategy to prepare XOS efficiently with simple operation steps, and XOS-rich hydrolysates could be potentially used as feed additives without NaHSO₄ separation.