Related Experiment Video
Updated: Sep 24, 2025
Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
Molecular detection of Orientia tsutsugamushi strains circulating in Nagaland
Siraj A Khan1, Jahnabi Saikia1, Trishna Bora1
1Medical Entomology, Arbovirology and Rickettsial Disease Division, Indian Council of Medical Research (ICMR)-Regional Medical Research Centre (RMRC), Northeast Region, Dibrugarh, 786010, Assam, India.
Abstract:
The antigenic heterogeneity of Orientia in India is still unknown in many disease endemic areas. The present study aims to characterize the strains of O. tsutsugamushi circulating in Nagaland, Northeast India. Two patients clinically diagnosed with ST and hospitalized in Mon district hospital, Nagaland were identified from whom eschar tissues were collected. Both patients demonstrated antibodies against O. tsutsugamushi along with positive PCR amplification for 56 kDa gene. The prototype strain TA763 shared 90.4% homology with the sequences. Both the sequences formed a distinctive cluster demonstrating 100% similarity with strains identified from Thailand, Vietnam, China and southern parts of India.
