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Updated: Sep 24, 2025

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
Published on: November 3, 2020
Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy
Federica Nardi1,2, Laura Pezzella1, Roberta Drago1,2
1Toscana Life Sciences Foundation, Siena, Italy.
Abstract:
Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.
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