Self-assembled quantum dot microstructure guided by a microemulsion approach for immunoassays

Quantum dot microstructures were fabricated through a convenient microemulsion approach in this study. A polymer solution containing a stabilizer was mixed with a quantum dot aqueous solution, to prepare a reversed microemulsion, through shaking. Then, the microemulsion was cast on a solid substrate followed by evaporating steps, resulting in the formation of an ordered porous film. Interestingly, the quantum dot microstructure can be produced at the same time. The immunoassay experiment could be realized by the fluorescent microstructures. The green fluorescence microstructure specifically bound with antigens marked with red color quantum dots, resulting in the enhancement of red fluorescence domains and the decrease of green fluorescence. With the addition of unlabeled antigens, the green fluorescence microstructure was recovered. This strategy implies that the quantum dot pattern has potential on biochip, biosensor, and imaging analysis.