Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysregulated Intestinal Nutrient Absorption in Obesity Is Associated with Altered Chromatin Accessibility.

Organoids·2025
Same author

Mesenchymal WNT2B is required for the development and function of the human intestine.

bioRxiv : the preprint server for biology·2025
Same author

Breast pericytes: a newly identified driver of tumor cell proliferation.

Frontiers in oncology·2025
Same author

Histologic and Endoscopic Findings Are Highly Correlated in a Prospective Cohort of Patients With Inflammatory Bowel Diseases.

Journal of Crohn's & colitis·2024
Same author

Host-derived CEACAM-laden vesicles engage enterotoxigenic <i>Escherichia coli</i> for elimination and toxin neutralization.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Comprehensive identification, isolation, and culture of human breast cell types.

The Journal of biological chemistry·2024

Related Experiment Video

Updated: Sep 24, 2025

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

974

Ulcerative Colitis: Novel Epithelial Insights Provided by Single Cell RNA Sequencing.

Joao M Serigado1, Jennifer Foulke-Abel2, William C Hines3

  • 1Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, United States.

Frontiers in Medicine
|May 9, 2022
PubMed
Summary

Single cell RNA sequencing reveals new insights into ulcerative colitis (UC) pathogenesis. This review highlights key findings on epithelial cell subpopulations, offering a framework for future research in gastrointestinal diseases.

Keywords:
Ulcerative Colitiscolonic microfold cellsgoblet cellsintestinal epitheliumsingle cell RNA sequencingstem cells

More Related Videos

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.6K
Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining
08:51

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

Published on: April 21, 2023

3.6K

Related Experiment Videos

Last Updated: Sep 24, 2025

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

974
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.6K
Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining
08:51

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

Published on: April 21, 2023

3.6K

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Immunology

Background:

  • Ulcerative colitis (UC) is a chronic intestinal inflammatory disease with unknown etiology.
  • Genetic and environmental factors contribute to UC development.
  • Single cell RNA sequencing (scRNA-seq) offers new insights into UC pathogenesis.

Purpose of the Study:

  • To review key scRNA-seq findings in epithelial cells of UC patients from two major studies.
  • To focus on specific cell subpopulations: BEST4+ cells, colonic M cells, goblet cells, colonocytes, and stem cells.
  • To compare methodologies, results, and conclusions between the studies and offer explanations.

Main Methods:

  • Analysis of scRNA-seq data from two independent UC studies.
  • Transcriptomic profiling of epithelial cell subpopulations.
  • Comparative analysis of methodologies and findings.

Main Results:

  • Identification of BEST4+ cells and colonic M cells in UC.
  • Detailed transcriptomic comparison of goblet cells, colonocytes, and stem cells in health and disease.
  • Validation of cell cluster markers and identification of commonalities and differences between studies.

Conclusions:

  • scRNA-seq provides a detailed view of the transcriptomic landscape in UC epithelial cells.
  • The findings offer a framework for the scientific and clinical GI community.
  • Further research can replicate or corroborate these extensive new findings.