Novel cationic tannin/glycosaminoglycan-based polyelectrolyte multilayers promote stem cells adhesion and...

Area of Science:

• Biomaterials Science
• Surface Chemistry
• Cell Biology

Background:

• Biocompatible surface coatings are crucial for controlling cellular interactions with biomaterials.
• Tailoring surface properties influences cell behavior, impacting applications in regenerative medicine and medical devices.

Purpose of the Study:

• To create cytocompatible coatings using layer-by-layer assembly of tanfloc (TN) with heparin (HEP) and chondroitin sulfate (CS).
• To investigate the effects of these polyelectrolyte multilayers (PEMs) on human adipose-derived stem cells (ADSCs) in vitro.
• To determine how surface charge and terminal layer composition affect stem cell responses.

Main Methods:

• Layer-by-layer (LbL) assembly was used to construct polyelectrolyte multilayers (PEMs).
• Surface plasmon resonance, X-ray photoelectron spectroscopy, atomic force microscopy, and contact angle measurements were employed for characterization.
• In vitro studies assessed the adhesion, proliferation, and spreading of ADSCs on the developed coatings.

Main Results:

• All TN-containing PEMs demonstrated cytocompatibility, supporting ADSC adhesion, proliferation, and spreading.
• The HEP-TN 11-layer assembly (HEP-TN11) exhibited the highest rate of cell proliferation.
• PEMs with TN as the terminal layer significantly promoted ADSC spreading, highlighting the role of surface charge and layer composition.

Conclusions:

• Tanfloc-based PEMs offer promising cytocompatible surface coatings for biomaterials.
• Surface properties, specifically the terminal layer and charge, critically influence stem cell behavior.
• These findings support the potential use of these coatings in biomedical implants and regenerative medicine applications.