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Published on: October 17, 2012
Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle
Siying Li1, Yangang Pan2, Honggang Teng2
1School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun, 130012 P. R. China.
Abstract:
The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 µm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.
Electronic Supplementary Material:
Supplementary material is available in the online version of this article at 10.1007/s40242-022-2069-y.
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