Detection of toxic choline based on Mn₂O₃/NiO nanomaterials by an electrochemical method

In this study, a novel in situ choline sensor was assembled by attaching the binary Mn₂O₃/NiO nanoparticles (NPs) onto a glassy carbon electrode (GCE). Initially, Mn₂O₃/NiO NPs were synthesized via a wet-chemical process and fully characterized via XRD, XPS, FESEM, EDS, FTIR and UV-Vis methods. The analytical performances of the choline sensor were evaluated by an electrochemical method in the phosphate buffer phase. The estimated linear dynamic range (LDR) was found to be 0.1 nM to 0.1 mM. The other analytical performances of the choline sensor, such as sensitivity (16.4557 μA μM^-1 cm^-2) and detection limit (5.77 ± 0.29 pM), were also calculated very carefully from the calibration plot. Overall, the choline sensor exhibited a reliable reproducibility, in situ validity, selectivity, interference effect, stability, and intra-day and inter-day performances with high accuracy in a short response time. Moreover, the probe was successfully applied to detect choline in real human, mouse and rabbit serum. This fabrication route would be a novel approach for the detection of selective biochemical sensor in the healthcare and biomedical fields.