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Cloning and nucleotide sequence of the chlD locus
Journal of Bacteriology
|May 1, 1987
Summary
Researchers identified genes in Escherichia coli responsible for transporting molybdate. The study reveals two open reading frames (ORFs) involved in active transport systems, suggesting chlD is the molybdate transport operon.
Area of Science:
- Molecular Biology
- Microbiology
- Genetics
Background:
- The chlD locus in Escherichia coli is implicated in nutrient transport.
- Understanding bacterial transport systems is crucial for microbial physiology.
Purpose of the Study:
- To determine the nucleotide sequence of a DNA fragment complementing an Escherichia coli chlD mutant.
- To identify genes involved in the chlD phenotype and elucidate their function.
Main Methods:
- Nucleotide sequencing of a Sau3A1 restriction nuclease fragment.
- DNA and deduced amino acid sequence analysis.
- Homology searches for conserved protein domains.
Main Results:
- Two open reading frames (ORFs) were identified within the sequenced fragment.
- One ORF encoded a hydrophobic inner membrane protein.
- The second ORF, responsible for complementing the chlD mutation, showed homology to nucleotide-binding proteins and hydrophilic inner membrane proteins involved in active transport.
Conclusions:
- The identified genes are likely involved in an active transport system.
- Evidence supports the proposal that the chlD locus functions as the molybdate transport operon in Escherichia coli.