Oncostatin M promotes lipolysis in white adipocytes

Related Experiment Video

Updated: Sep 24, 2025

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

3.1K

Pim P van Krieken1,2, Julian Roos3, Pamela Fischer-Posovszky3

  • 1Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, University of Zurich, Zurich, Switzerland.

Adipocyte
|May 9, 2022
PubMed

Insights

Oncostatin M (OSM) directly promotes lipolysis in white adipocytes by activating hormone-sensitive lipase (HSL) via ERK1/2 signaling. This finding clarifies OSM's complex role in metabolic regulation and insulin resistance.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Oncostatin M (OSM), a glycoprotein 130 cytokine, is implicated in chronic inflammation and metabolic dysfunction.
  • OSM's role in adipocytes is complex, with inhibitory effects on adipogenesis and browning, yet potentially favorable roles in obesity models.

Purpose of the Study:

  • To investigate the role of Oncostatin M (OSM) in modulating lipolysis in white adipocytes.
  • To elucidate the molecular mechanisms underlying OSM-induced lipolysis.

Main Methods:

  • Primary and cell line-derived white adipocytes (murine and human) were treated with OSM.
  • Assays included measurement of lipolysis markers (free fatty acids, glycerol), hormone-sensitive lipase (HSL) activity, and protein phosphorylation (IRS1, ERK1/2).
  • Specific inhibitors of extracellular signal-regulated kinase (ERK)-1/2 were used to assess pathway involvement.

Main Results:

  • OSM stimulation directly activated hormone-sensitive lipase (HSL) and increased lipolysis in a dose-dependent manner.
  • OSM attenuated insulin's suppressive effect on lipolysis and induced insulin receptor substrate-1 (IRS1) phosphorylation.
  • Inhibition of ERK1/2 activation abolished OSM-mediated HSL phosphorylation and lipolysis.

Conclusions:

  • Oncostatin M signaling directly promotes lipolysis in white adipocytes.
  • This effect is mediated through the extracellular signal-regulated kinase (ERK)-1/2 pathway.
  • OSM's pro-lipolytic action contributes to its complex role in metabolic regulation and insulin resistance.
Keywords:
Adipocytecytokineglycoprotein 130insulin resistancelipolysisoncostatin M

More Related Videos

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads07:59

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Published on: July 21, 2017

9.8K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.4K

Related Concept Videos

Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
198
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
94.3K
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
10.0K
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
2.7K
Lipid Absorption01:24

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
884