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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

455
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Sep 24, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis.

Charlotte Chambers1, Linh Quan1, Grace Yi1

  • 1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School.

Journal of Visualized Experiments : Jove
|May 9, 2022
PubMed
Summary

This study uses CRISPR gene editing to investigate clustered microRNAs (miRNAs) in prostate cancer. The high-throughput method efficiently deletes miRNA clusters, revealing their cooperative roles in cancer progression and drug resistance.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • MicroRNAs (miRNAs) are key regulators in cancer, acting as tumor suppressors or oncogenes.
  • Most research examines single miRNAs, overlooking co-expressed miRNA clusters (~30% of human miRNA genes).
  • Understanding clustered miRNA networks is crucial for cancer therapy development.

Purpose of the Study:

  • To investigate the oncogenic role of a seven-miRNA gene cluster in prostate cancer.
  • To develop and utilize a high-throughput CRISPR-mediated gene editing system for studying miRNA clusters.
  • To uncover cooperative interactions within miRNA clusters and their impact on cancer.

Main Methods:

  • Employed doxycycline-inducible Cas9 in human cancer cell lines.
  • Used lentiviral vectors and CRISPR RNA (crRNA) with trans-activating CRISPR RNA (tracrRNA) for gene editing.
  • Generated cell lines with whole or partial miRNA cluster deletions for functional analysis.

Main Results:

  • Successfully created cancer cell lines with specific miRNA cluster deletions.
  • Demonstrated the feasibility of high-throughput deletion of miRNA gene clusters.
  • Established a streamlined method for studying miRNA cluster function.

Conclusions:

  • This CRISPR-based approach enables efficient study of miRNA cluster function and interactions.
  • Findings will elucidate the complex roles of small noncoding RNA networks in cancer.
  • Informed future therapeutic strategies targeting miRNA clusters in disease.