Related Experiment Video
Updated: Sep 24, 2025
The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice
Published on: November 1, 2015
A novel lineage-tracing mouse model for studying early MmuPV1 infections
Vural Yilmaz1, Panayiota Louca1, Louiza Potamiti2
1Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus.
Abstract:
Human papillomaviruses are DNA viruses that ubiquitously infect humans and have been associated with hyperproliferative lesions. The recently discovered mouse specific papillomavirus (MmuPV1) provides the opportunity to study papillomavirus infections in vivo in the context of a common laboratory mouse model (Mus musculus). To date, a major challenge in the field has been the lack of tools to identify, observe, and characterize individually the papillomavirus hosting cells and also trace the progeny of these cells over time. Here, we present the successful generation of an in vivo lineage-tracing model of MmuPV1-harboring cells and their progeny by means of genetic reporter activation. Following the validation of the system both in vitro and in vivo, we used it to provide a proof-of-concept of its utility. Using flow-cytometry analysis, we observed increased proliferation dynamics and decreased MHC-I cell surface expression in MmuPV1-treated tissues which could have implications in tissue regenerative capacity and ability to clear the virus. This model is a novel tool to study the biology of the MmuPV1 host-pathogen interactions.
Insights
Researchers developed a new lineage-tracing model to study mouse papillomavirus (MmuPV1) infections in vivo. This tool tracks virus-hosting cells and their progeny, revealing insights into MmuPV1-host interactions and immune responses.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Human papillomaviruses (HPVs) cause hyperproliferative lesions.
- Mouse papillomavirus (MmuPV1) offers an in vivo model for HPV studies in *Mus musculus*.
- Lack of tools hinders tracking of MmuPV1-infected cells and their progeny.
Purpose of the Study:
- To develop and validate an in vivo lineage-tracing model for MmuPV1-harboring cells.
- To utilize the model for studying MmuPV1-host interactions.
- To investigate the impact of MmuPV1 on cell proliferation and immune evasion.
Main Methods:
- Generation of a genetic reporter system for lineage tracing.
- In vitro and in vivo validation of the reporter system.
- Flow-cytometry analysis of MmuPV1-treated tissues.
Main Results:
- Successful generation of an in vivo lineage-tracing model for MmuPV1.
- Observed increased proliferation dynamics in MmuPV1-treated tissues.
- Detected decreased MHC-I cell surface expression on MmuPV1-affected cells.
Conclusions:
- The novel lineage-tracing model enables detailed study of MmuPV1-host interactions.
- Findings suggest MmuPV1 influences tissue regeneration and immune evasion.
- The model provides a valuable tool for understanding papillomavirus pathogenesis.
