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Updated: Sep 24, 2025
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
Published on: March 16, 2022
Generation of a human embryonic stem cell line (WAe009-A-78) carrying homozygous TBX18 knockout
Qi Xu1, Tiantian Zhao2, Yongshuai Zhang1
1State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Key Laboratory of Application of Pluripotent Stem Cells in Heart Regeneration, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Abstract:
T-Box Transcription Factor 18 is a member of the T-box family, encoding TBX18 protein. As a transcriptional repressor, it related to developmental processes of a majority of tissues and organs and plays crucial part in the embryonic development of sinoatrial node. Using an episomal vector-based CRISPR/Cas9 system, we have established a homozygous TBX18 knockout (TBX18-KO) human embryonic stem cell (hESC) line. This newly TBX18-/- hESC line display normal pluripotency, morphology, karyotype and trilineage differentiating capacity. This cell line may provide a powerful tool to investigate the role of TBX18 gene in sinoatrial node development in future.
